SBI Lentiviral Technologies

SBI Lentiviral Technologies pGF2 mcmv

Ülevaade

Tehke oma isiklik pGreenFire 2.zero reportervektor või kasutage seda halva haldusena

Tänu pGreenFire 2.zero mCMV kloonimisele ja ebasoodsale juhtimisele ja viirusele (pGF2-mCMV-rFluc-T2A-GFP-mPGK-Puro) saate maksimaalselt kasutada meie tugevat pGreenFire 2.zero lentivector oskusteavet, et luua oma isiklik transkriptsioonivastuse komponendi (TRE) reporterit või kasutage kahjuliku haldusena pGreenFire 2.zero mCMV.

PGreenFire 2.zero mCMV kloonimise ja ebasoodsa juhtimise lektoriga on XhoI ja NheI veebisaidid paigutatud minimaalse CMV promootori (mCMV) eelvoolu, nii et saate kloonida oma isiklikesse TRE -desse. Aktiveerimisel juhivad TRE-d ja mCMV promootor ühiselt karmiinpunase lillesiferaasi ja GFP koosekspressiooni, nii et saate kvantitatiivselt mõõta transkriptsioonilist harjutust, kasutades iga fluorestsents- ja lutsiferaasiharjutust.

Teise võimalusena peate kasutama vektorit kahjuliku haldusena iga ettevõtja jaoks, kes kasutab pGreenFire 2.zero lentivectorsi (vt teavet allpool).

Mis muudab meie järgmise põlvkonna pGreenFire 2.zero vektorid isegi kõrgemaks kui erinevad TRE reportervektorid, on hea disain, mis pakub konstantse valiku kassetti stabiilse rakuliini ajastu jaoks, minimeerides samas häireid ülesvoolu TRE-s. Kasutades nõrka/mõistlikku mPGK promootorit antibiootikumide valiku markeri (puromütsiiniresistentsus) juhtimiseks ja tingimuslike reportergeenide põhjalikku korraldamist, väljendatakse valikumarkerit usaldusväärselt ilma rFLuc ja GFP kahjustava tingimusliku ekspressioonita.

UUE TOOTE HOIATUS!

SBI töötab välja, et teatada meie järgneva ajastu pGreenFire signaaliraja reporterite käivitamisest! Oleme täiustanud neid standardseid laenuvõtjaid intelligentse disainiga, mis võimaldab usaldusväärset rakujälgede ajastu, ning oleme lisaks muutnud traditsioonilist lutsiferaasi reporterit karmiinpunase lillesiferaasiga, mis avab kahespektrilise lutsiferaasi testi teostamise potentsiaali ja suurendab tundlikkust  in vivo  funktsioone kui tüüpiline lutsiferaas.

SBI Lentiviral Technologies
SBI Lentiviral Technologies

pGreenFire põhialused

Neile, kes pole meie pGreenFire reporteritega tuttavad, on kõigil 1. nullil ja paaril nullil analoogne põhitulemus- transkriptsioonivastuse osad (TRE) on paigutatud minimaalse CMV promootori (mCMV) ja pGreenFire lutsiferaasi- T2A-GFP koekspressioonikassett. Transkriptsiooni aktiveerimise puudumisel on mCMV promootoril tühine treening, mis põhjustab vähese või nutsutsiferaasi kasutamise või GFP fluorestsentsi (1. määramine). Sellegipoolest, TRE-de aktiveerimisel, mis vastab vastuseks indutseerija lisamisele, juhivad TRE-d pluss mCMV promootor iga lutsiferaasi ja GFP ekspressiooni annusest sõltuvas trendis (1. määramine). Tulemuseks on paindlikkus raja aktiveerimise kvantitatiivseks mõõtmiseks, kasutades lutsiferaasi või GFP -d.

Nagu kõigi meie pGreenFire 2.zero lentivektorite puhul, koosneb ka GreenFire kassett nüüd karmiinpunase tulekese lutsiferaasist (rFLuc), T2A koekspressioonikomponendist ja GFP-st. Üleminek rFLuc-le avab kahespektrilise lutsiferaasi testi teostamise potentsiaali ja annab samuti suurema tundlikkuse  in vivo  funktsioonide suhtes kui tüüpiline lutsiferaas.

 

Anti-Anti-SEPT2 Antibody antibody

STJ25475 100 µl
EUR 277

Anti-Anti-SEPT5 Antibody antibody

STJ25477 100 µl
EUR 277
Description: This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced.

Anti-Anti-SEPT8 Antibody antibody

STJ25479 100 µl
EUR 277
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Anti-Anti-SEPT2 Antibody antibody

STJ28365 100 µl
EUR 277

Anti-Anti-SEPT7 Antibody antibody

STJ28963 100 µl
EUR 277
Description: This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19.

Anti-Anti-SEPT8 Antibody antibody

STJ117206 100 µl
EUR 277
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Anti-Anti-SEPT12 Antibody antibody

STJ117759 100 µl
EUR 277
Description: This gene encodes a guanine-nucleotide binding protein and member of the septin family of cytoskeletal GTPases. Septins play important roles in cytokinesis, exocytosis, embryonic development, and membrane dynamics. Multiple transcript variants encoding different isoforms have been found for this gene.

Anti-Anti-MARCH6 Antibody antibody

STJ118549 100 µl
EUR 277

Anti-Anti-MARCH6 Antibody antibody

STJ118550 100 µl
EUR 277

Anti-Anti-MARCH7 Antibody antibody

STJ118752 100 µl
EUR 277

Anti-Anti-SEPT3 Antibody antibody

STJ118990 100 µl
EUR 277

Anti-Anti-SEPT1 antibody antibody

STJ119580 100 µl
EUR 277
Description: This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis and the maintenance of cellular morphology. This gene encodes a protein that can form homo- and heterooligomeric filaments, and may contribute to the formation of neurofibrillary tangles in Alzheimer's disease. Alternatively spliced transcript variants have been found but the full-length nature of these variants has not been determined. [provided by RefSeq, Dec 2012]

Anti-Anti-SEPT7 Antibody antibody

STJ116214 100 µl
EUR 277
Description: This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19.

Anti-Anti-SEPT6 antibody antibody

STJ11100949 100 µl
EUR 277
Description: This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis. One version of pediatric acute myeloid leukemia is the result of a reciprocal translocation between chromosomes 11 and X, with the breakpoint associated with the genes encoding the mixed-lineage leukemia and septin 2 proteins. This gene encodes four transcript variants encoding three distinct isoforms. An additional transcript variant has been identified, but its biological validity has not been determined.

Anti-Anti-SEPT9 Antibody antibody

STJ111369 100 µl
EUR 277
Description: This gene is a member of the septin family involved in cytokinesis and cell cycle control. This gene is a candidate for the ovarian tumor suppressor gene. Mutations in this gene cause hereditary neuralgic amyotrophy, also known as neuritis with brachial predilection. A chromosomal translocation involving this gene on chromosome 17 and the MLL gene on chromosome 11 results in acute myelomonocytic leukemia. Multiple alternatively spliced transcript variants encoding different isoforms have been described.

Anti-Anti-SEPT11 Antibody antibody

STJ111530 100 µl
EUR 277

Anti-Anti-SEPT4 Antibody antibody

STJ112276 100 µl
EUR 277
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is highly expressed in brain and heart. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. One of the isoforms (known as ARTS) is distinct; it is localized to the mitochondria, and has a role in apoptosis and cancer.

Anti-Anti-MARCH9 Antibody antibody

STJ112609 100 µl
EUR 277

Anti-Anti-SEPT11 Antibody antibody

STJ113941 100 µl
EUR 277

Anti-Anti-SEPT11 Antibody antibody

STJ114081 100 µl
EUR 277

Anti-Anti-SEPT5 Antibody antibody

STJ114819 100 µl
EUR 277
Description: This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced.

Anti-Anti-MARCH8 Antibody antibody

STJ114828 100 µl
EUR 277

Ly1 Antibody Reactive (LYAR) Antibody

20-abx123734
  • EUR 411.00
  • EUR 592.00
  • 100 ul
  • 200 ul

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