Peamised valikud ja üksikasjad
- Tundlikkus: 10 pg/ml
- Erinevus : 78 pg/ml – 5000 pg/ml
- Proovi tüüp : rakkude kohandatud supernatant, rakulüsaat, EDTA -plasma, hep -plasma, seerum
- Tuvastamine lähenemisviisi kolorimeetriline
- Analüüsi tüüp : võileib (kvantitatiivne)
- Reageerib järgmiselt: Inimene
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Toote loomine
Inimese BAFF-R ELISA tööriistad -
tuvastamine lähenemine
Kolorimeetriline -
Täpsus
Intra-test Näidis n Märkige SD CV% 1 16 147 pg/ml 10.29 = 7% 2 16 683 pg/ml 49,85 = 7,3% 3 16 2750 pg/ml 206,25 = 7,5% Analüüs Näidis n Märkige SD CV% 1 24 156 pg/ml 13,88 = 8,9% 2 24 700 pg/ml 62,3 = 8,9% 3 24 2531 pg/ml 227,05 = 8,9% -
Proovi tüüp
Rakkude kohandatud supernatant, seerum, rakulüsaat, hep -plasma, EDTA -plasma -
Analüüsi tüüp
Võileib (kvantitatiivne) -
Tundlikkus
<10 pg/ml -
Erinevad
78 pg/ml – 5000 pg/ml -
Analüüsi aeg
3h 30m -
Analüüsi suurus
Palju tavalisi samme -
Liigi reaktsioonivõime
Reageerib järgmiselt: Inimene
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Toote ülevaade
Inimese BAFF-R ensüümiga seotud immunosorbentanalüüsi (ELISA) käik (ab213839) on ette nähtud inimese TNFRSF13C kvantitatiivseks mõõtmiseks rakkude kohandatud supernatantides, rakulüsaatides, seerumis ja plasmas (hepariin, EDTA).
ELISA käik põhineb tavapärastel võileivaensüümidega seotud immuun-sorbendi testi kogemustel . Monokloonne antikeha hiire selgesõnalist BAFF-R on eelnevalt kanti 96-süvendiga plaatidele. Necessities (Expression süsteem regulaarsel : NSO; Immunogen järjestus: S7-A71) ja kontrollida proovid lisatakse aukudesse, biotinüülitud avastamis- antikehaga kitse selgesõnalist BAFF-R lisatakse hiljem misjärel vastuvõetud pesemist PBS või TBS puhver. Avidiin-biotiin-peroksidaas Keerukaslisatakse ja seondumata konjugaadid pestakse PBS- või TBS -puhvriga. HRP substraati TMB kasutatakse HRP ensümaatilise vastuse visualiseerimiseks. TMB katalüüsib HRP toota sinist tooni toode, mis lahkus pärast kollaseks koos happeliste stop vastuses . Kollase tihedus on võrdeline plaadile võetud proovi kogusega inimese BAFF-R .
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Märkused
BAFF-R ( lisaks tuntakse Tuumornekroosifaktor väljakutse ülemsugukonda liikme 13C või B-rakkude aktiveeriv väljakutse retseptor) on valk, mis on inimestel on kodeeritud TNFRSF13C geen. Homoloogides BAC klooniga kaardistati BAFFR geen kromosoomile 22q13.1-q13.31. B-raku aktiveeriv väljakutse (BAFF) suurendab B-rakkude ellujäämist in vitro ja on perifeersete B-rakkude elanike regulaator. Selle geeni kodeeritud valk on BAFF-i retseptor ja see on III vormi transmembraanne valk, mis sisaldab ühte rakuvälist fenüülalaniinirikast ruumi . see on arvasin, et see retseptor on peamine retseptor, mis on vajalik BAFF-vahendatud küpsete B-rakkude ellujäämiseks.
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Platvorm
qayee bio human baff r
Human BAFF (BLyS) Receptor, (Recombinant) |
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| 22060255-1 | Bio-WORLD | 5 µg | EUR 146.42 |
BAFF-R, Human |
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| HY-P7130 | MedChemExpress | 10ug | EUR 183.6 |
Human B-Cell Activating Factor(BAFF)ELISA Kit |
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| QY-E05096 | Qayee Biotechnology | 96T | EUR 433.2 |
Human B cell activation factorr from the tumor necrosis factor family receptor(BAFF-R)ELISA Kit |
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| QY-E05097 | Qayee Biotechnology | 96T | EUR 433.2 |
Human BAFF-R(CD268) |
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| 90103-A | BPS Bioscience | 10 µg | EUR 130 |
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Description: Recombinant human BAFF-R (B cell-activating factor from the TNF family), also known as CD268, is a disulfide-linked monomeric protein consisting of 76 a.a. and migrates as an approximately 9 kDa protein under reducing and non-reducing conditions. Optimized DNA sequence encoding human BAFF-R extracellular domain was expressed in E. coli. |
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Human BAFF-R(CD268) |
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| 90103-B | BPS Bioscience | 50 µg | EUR 205 |
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Description: Recombinant human BAFF-R (B cell-activating factor from the TNF family), also known as CD268, is a disulfide-linked monomeric protein consisting of 76 a.a. and migrates as an approximately 9 kDa protein under reducing and non-reducing conditions. Optimized DNA sequence encoding human BAFF-R extracellular domain was expressed in E. coli. |
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Human B cell activating factor of TNF family(BAFF)ELISA Kit |
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| QY-E04538 | Qayee Biotechnology | 96T | EUR 433.2 |
BAFF (B-cell Activating Factor) |
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| RC214-15 | Bio Basic | 5ug | EUR 125.26 |
Baff receptor Antibody / Baff-r |
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| RQ4338 | NSJ Bioreagents | 100 ug | EUR 356.15 |
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Description: Tumor necrosis factor receptor superfamily member 13C (TNFRSF13C), also known as BAFFR, is a protein in humans is encoded by the TNFRSF13C gene. The BAFFR gene is mapped to chromosome 22q13.1-q13.31. It has got 184 amino acid transmembrane protein which is 56% identical to the mouse protein. B cell-activating factor (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. BAFF plays a crucial role in B cell development and can function through receptors other than BCMA. |
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BAFF Receptor Antibody / BAFF-R |
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| RQ4381 | NSJ Bioreagents | 100 ug | EUR 356.15 |
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Description: Tumor necrosis factor receptor superfamily member 13C (TNFRSF13C), also known as BAFFR, is a protein in humans is encoded by the TNFRSF13C gene. The BAFFR gene is mapped to chromosome 22q13.1-q13.31. It has got 184 amino acid transmembrane protein which is 56% identical to the mouse protein. B cell-activating factor (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. BAFF plays a crucial role in B cell development and can function through receptors other than BCMA. |
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Rat B-Cell Activating Factor(BAFF)ELISA Kit |
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| QY-E10385 | Qayee Biotechnology | 96T | EUR 433.2 |
Mouse B-Cell Activating Factor(BAFF)ELISA Kit |
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| QY-E20186 | Qayee Biotechnology | 96T | EUR 433.2 |
BAFF-R Antibody |
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| 3578R-100 | Biovision | each | EUR 379.2 |
BAFF-R Antibody |
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| 3578R-30T | Biovision | each | EUR 175.2 |
BAFF-R Antibody |
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| R30462 | NSJ Bioreagents | 100 ug | EUR 356.15 |
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Description: Tumor necrosis factor receptor superfamily member 13C (TNFRSF13C), also known as BAFF-R, is a protein in humans is encoded by the TNFRSF13C gene. The gene is mapped to chromosome 22q13.1-q13.31. It has got 184 amino acid transmembrane protein which is 56% identical to the mouse protein. B cell-activating factor enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. BAFF plays a crucial role in B cell development and can function through receptors other than BCMA. |
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Human BAFF-R Protein, mFc Tag |
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| E33P100044 | EnoGene | 10 μg | EUR 225 |
Human BAFF-R Protein, hFc Tag |
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| E33P100503 | EnoGene | 10 μg | EUR 225 |
BAFF-R Blocking Peptide |
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| 3578RBP-50 | Biovision | each | EUR 183.6 |
Polyclonal BAFF-R Antibody |
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| APR15125G | Leading Biology | 0.1mg | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human BAFF-R . This antibody is tested and proven to work in the following applications: |
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BAFF-R Polyclonal Antibody |
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| 3578-100 | Biovision | each | EUR 379.2 |
BAFF-R Recombinant Protein |
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| 90-237 | ProSci | 50 ug | EUR 600.9 |
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Description: BAFF is mainly produced by innate immune cells such as neutrophils, monocytes, macrophages, dendritic cells, follicular dendritic cells. T cells, activated B cells, some malignant B cells and also non-lymphoid cells like astrocytes, synoviocytes and epithelial cells can also produce BAFF. BAFF binds three distinct receptors (BAFF-R, TACI and BCMA) expressed predominantly on B cells, although activated T cells also express BAFF-R. BAFF is a master regulator of peripheral B cell survival, and together with IL-6, promotes Ig class-switching and plasma cell differentiation . Besides its major role in B cell biology, BAFF co-stimulates activated T cells. Deregulated expression of BAFF leads to autoimmune disorders in mice. In humans, elevated levels of soluble BAFF have been detected in the serum of patients with various autoimmune diseases, such as Sjögren’s syndrome , Rheumatoid Arthritis (RA) , Multiple sclerosis (MS) and Systemic Lupus Erythematosus (SLE) . BAFF is also increased levels in some lymphoid cancers. |
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Biotinylated Recombinant Human TNFRSF13C/BAFF-R/CD268 Protein |
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| RP02582 | Abclonal | 1000μg | EUR 422.5 |
PE anti-human CD268 (BAFF-R) |
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| E16FHP268-025 | EnoGene | 25 tests | EUR 476.67 |
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Description: Available in various conjugation types. |
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PE anti-human CD268 (BAFF-R) |
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| E16FHP268-100 | EnoGene | 100 tests | EUR 1083.33 |
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Description: Available in various conjugation types. |
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Antibody Bio-Stabilizer 10X |
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| 22050005-1 | Bio-WORLD | 20 mL | EUR 49.93 |
Antibody Bio-Stabilizer 10X |
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| 22050005-2 | Bio-WORLD | 50 mL | EUR 85.98 |