Peamised valikud ja üksikasjad
- Tundlikkus: 10 pg/ml
- Erinevus : 78 pg/ml – 5000 pg/ml
- Proovi tüüp : rakkude kohandatud supernatant, rakulüsaat, EDTA -plasma, hep -plasma, seerum
- Tuvastamine lähenemisviisi kolorimeetriline
- Analüüsi tüüp : võileib (kvantitatiivne)
- Reageerib järgmiselt: Inimene
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Toote loomine
Inimese BAFF-R ELISA tööriistad -
tuvastamine lähenemine
Kolorimeetriline -
Täpsus
Intra-test Näidis n Märkige SD CV% 1 16 147 pg/ml 10.29 = 7% 2 16 683 pg/ml 49,85 = 7,3% 3 16 2750 pg/ml 206,25 = 7,5% Analüüs Näidis n Märkige SD CV% 1 24 156 pg/ml 13,88 = 8,9% 2 24 700 pg/ml 62,3 = 8,9% 3 24 2531 pg/ml 227,05 = 8,9% -
Proovi tüüp
Rakkude kohandatud supernatant, seerum, rakulüsaat, hep -plasma, EDTA -plasma -
Analüüsi tüüp
Võileib (kvantitatiivne) -
Tundlikkus
<10 pg/ml -
Erinevad
78 pg/ml – 5000 pg/ml -
Analüüsi aeg
3h 30m -
Analüüsi suurus
Palju tavalisi samme -
Liigi reaktsioonivõime
Reageerib järgmiselt: Inimene
-
Toote ülevaade
Inimese BAFF-R ensüümiga seotud immunosorbentanalüüsi (ELISA) käik (ab213839) on ette nähtud inimese TNFRSF13C kvantitatiivseks mõõtmiseks rakkude kohandatud supernatantides, rakulüsaatides, seerumis ja plasmas (hepariin, EDTA).
ELISA käik põhineb tavapärastel võileivaensüümidega seotud immuun-sorbendi testi kogemustel . Monokloonne antikeha hiire selgesõnalist BAFF-R on eelnevalt kanti 96-süvendiga plaatidele. Necessities (Expression süsteem regulaarsel : NSO; Immunogen järjestus: S7-A71) ja kontrollida proovid lisatakse aukudesse, biotinüülitud avastamis- antikehaga kitse selgesõnalist BAFF-R lisatakse hiljem misjärel vastuvõetud pesemist PBS või TBS puhver. Avidiin-biotiin-peroksidaas Keerukaslisatakse ja seondumata konjugaadid pestakse PBS- või TBS -puhvriga. HRP substraati TMB kasutatakse HRP ensümaatilise vastuse visualiseerimiseks. TMB katalüüsib HRP toota sinist tooni toode, mis lahkus pärast kollaseks koos happeliste stop vastuses . Kollase tihedus on võrdeline plaadile võetud proovi kogusega inimese BAFF-R .
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Märkused
BAFF-R ( lisaks tuntakse Tuumornekroosifaktor väljakutse ülemsugukonda liikme 13C või B-rakkude aktiveeriv väljakutse retseptor) on valk, mis on inimestel on kodeeritud TNFRSF13C geen. Homoloogides BAC klooniga kaardistati BAFFR geen kromosoomile 22q13.1-q13.31. B-raku aktiveeriv väljakutse (BAFF) suurendab B-rakkude ellujäämist in vitro ja on perifeersete B-rakkude elanike regulaator. Selle geeni kodeeritud valk on BAFF-i retseptor ja see on III vormi transmembraanne valk, mis sisaldab ühte rakuvälist fenüülalaniinirikast ruumi . see on arvasin, et see retseptor on peamine retseptor, mis on vajalik BAFF-vahendatud küpsete B-rakkude ellujäämiseks.
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Platvorm
qayee bio human baff r
Human BAFF (BLyS) Receptor, (Recombinant) |
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| 22060255-1 | Bio-WORLD | 5 µg | EUR 190.94 |
BAFF-R, Human |
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| HY-P7130 | MedChemExpress | 10ug | EUR 183.6 |
BAFF (B-cell Activating Factor) |
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| RC214-15 | Bio Basic | 5ug | EUR 125.26 |
Human BAFF-R(CD268) |
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| 90103-A | BPS Bioscience | 10 µg | EUR 130 |
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Description: Recombinant human BAFF-R (B cell-activating factor from the TNF family), also known as CD268, is a disulfide-linked monomeric protein consisting of 76 a.a. and migrates as an approximately 9 kDa protein under reducing and non-reducing conditions. Optimized DNA sequence encoding human BAFF-R extracellular domain was expressed in E. coli. |
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Human BAFF-R(CD268) |
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| 90103-B | BPS Bioscience | 50 µg | EUR 205 |
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Description: Recombinant human BAFF-R (B cell-activating factor from the TNF family), also known as CD268, is a disulfide-linked monomeric protein consisting of 76 a.a. and migrates as an approximately 9 kDa protein under reducing and non-reducing conditions. Optimized DNA sequence encoding human BAFF-R extracellular domain was expressed in E. coli. |
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Recombinant Human BAFF-R |
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| MBS7611153-005mg | MyBiosource | 0.05mg | EUR 345 |
Recombinant Human BAFF-R |
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| MBS7611153-02mg | MyBiosource | 0.2mg | EUR 635 |
Recombinant Human BAFF-R |
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| MBS7611153-1mg | MyBiosource | 1mg | EUR 1800 |
Recombinant Human BAFF-R |
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| MBS7611153-5x1mg | MyBiosource | 5x1mg | EUR 6955 |
BAFF-R(human):Fc(human) |
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| BC-323 | Kamiya Biomedical Company | 50 ug | EUR 667 |
[Cys0]-BAFF-R (159-183) (Mouse) / [Cys0]-BAFF-R (151-175) (Human) |
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| 030-51 | PHOENIX PEPTIDE | 100 μg | EUR 177.12 |
Human BAFF-R (CD268) Recombinant |
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| MBS553135-001mg | MyBiosource | 0.01mg | EUR 165 |
Human BAFF-R (CD268) Recombinant |
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| MBS553135-005mg | MyBiosource | 0.05mg | EUR 245 |
[Cys0]-BAFF-R (159-183) (Mouse) / [Cys0]-BAFF-R (151-175) (Human) - Antibody |
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| H-030-51 | PHOENIX PEPTIDE | 50 μl | EUR 604.8 |
Recombinant Human BAFF-R (TNFRSF13C) |
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| MBS4159631-005mg | MyBiosource | 0.05mg | EUR 255 |
Recombinant Human BAFF-R (TNFRSF13C) |
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| MBS4159631-01mg | MyBiosource | 0.1mg | EUR 360 |
Recombinant Human BAFF-R (TNFRSF13C) |
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| MBS4159631-1mg | MyBiosource | 1mg | EUR 1335 |
Recombinant Human BAFF-R (TNFRSF13C) |
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| MBS4159631-5x1mg | MyBiosource | 5x1mg | EUR 5770 |
Recombinant Human BAFF-R (TNFRSF13C) |
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| Z100015 | ABM | 50 µg | EUR 105 |
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Description: The B cell-activating factor from the TNF family (BAFF), is emerging as an important regulator of B cell and T cell responses. BAFF was originally identified as a factor responsible for B cell survival and maturation. BAFF binds to several receptors. These include transmembrane activator and calcium modulator and cyclophilin ligand interactor (TACI), BAFF-R (BR3), and B cell maturation Ag (BCMA). BAFF-R appears to be particularly important for the regulation of B cell survival and maturation in the spleen, because A/WySnJ mice expressing a defective BAFF-R have disrupted B cell maturation, similar to that seen in BAFF-deficient mice. |
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Recombinant Human BAFF-R (TNFRSF13C) |
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| Z100017 | ABM | 100 µg | EUR 215 |
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Description: The B cell-activating factor from the TNF family (BAFF), is emerging as an important regulator of B cell and T cell responses. BAFF was originally identified as a factor responsible for B cell survival and maturation. BAFF binds to several receptors. These include transmembrane activator and calcium modulator and cyclophilin ligand interactor (TACI), BAFF-R (BR3), and B cell maturation Ag (BCMA). BAFF-R appears to be particularly important for the regulation of B cell survival and maturation in the spleen, because A/WySnJ mice expressing a defective BAFF-R have disrupted B cell maturation, similar to that seen in BAFF-deficient mice. |
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Recombinant Human BAFF-R (TNFRSF13C) |
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| Z100019 | ABM | 1.0 mg | EUR 1060 |
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Description: The B cell-activating factor from the TNF family (BAFF), is emerging as an important regulator of B cell and T cell responses. BAFF was originally identified as a factor responsible for B cell survival and maturation. BAFF binds to several receptors. These include transmembrane activator and calcium modulator and cyclophilin ligand interactor (TACI), BAFF-R (BR3), and B cell maturation Ag (BCMA). BAFF-R appears to be particularly important for the regulation of B cell survival and maturation in the spleen, because A/WySnJ mice expressing a defective BAFF-R have disrupted B cell maturation, similar to that seen in BAFF-deficient mice. |
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BAFF-R (human):Fc (human), (recombinant) |
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| MBS566224-005mg | MyBiosource | 0.05mg | EUR 540 |
BAFF-R (human):Fc (human), (recombinant) |
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| MBS566224-5x005mg | MyBiosource | 5x0.05mg | EUR 2375 |
BAFF Receptor, Recombinant, Human (BAFF-R, BR3, BLyS Receptor 3) |
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| MBS637358-0005mg | MyBiosource | 0.005mg | EUR 340 |
BAFF Receptor, Recombinant, Human (BAFF-R, BR3, BLyS Receptor 3) |
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| MBS637358-002mg | MyBiosource | 0.02mg | EUR 490 |
BAFF Receptor, Recombinant, Human (BAFF-R, BR3, BLyS Receptor 3) |
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| MBS637358-5x002mg | MyBiosource | 5x0.02mg | EUR 2055 |