Peamised valikud ja üksikasjad
- Tundlikkus: 10 pg/ml
- Erinevus : 78 pg/ml – 5000 pg/ml
- Proovi tüüp : rakkude kohandatud supernatant, rakulüsaat, EDTA -plasma, hep -plasma, seerum
- Tuvastamine lähenemisviisi kolorimeetriline
- Analüüsi tüüp : võileib (kvantitatiivne)
- Reageerib järgmiselt: Inimene
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Toote loomine
Inimese BAFF-R ELISA tööriistad -
tuvastamine lähenemine
Kolorimeetriline -
Täpsus
Intra-test Näidis n Märkige SD CV% 1 16 147 pg/ml 10.29 = 7% 2 16 683 pg/ml 49,85 = 7,3% 3 16 2750 pg/ml 206,25 = 7,5% Analüüs Näidis n Märkige SD CV% 1 24 156 pg/ml 13,88 = 8,9% 2 24 700 pg/ml 62,3 = 8,9% 3 24 2531 pg/ml 227,05 = 8,9% -
Proovi tüüp
Rakkude kohandatud supernatant, seerum, rakulüsaat, hep -plasma, EDTA -plasma -
Analüüsi tüüp
Võileib (kvantitatiivne) -
Tundlikkus
<10 pg/ml -
Erinevad
78 pg/ml – 5000 pg/ml -
Analüüsi aeg
3h 30m -
Analüüsi suurus
Palju tavalisi samme -
Liigi reaktsioonivõime
Reageerib järgmiselt: Inimene
-
Toote ülevaade
Inimese BAFF-R ensüümiga seotud immunosorbentanalüüsi (ELISA) käik (ab213839) on ette nähtud inimese TNFRSF13C kvantitatiivseks mõõtmiseks rakkude kohandatud supernatantides, rakulüsaatides, seerumis ja plasmas (hepariin, EDTA).
ELISA käik põhineb tavapärastel võileivaensüümidega seotud immuun-sorbendi testi kogemustel . Monokloonne antikeha hiire selgesõnalist BAFF-R on eelnevalt kanti 96-süvendiga plaatidele. Necessities (Expression süsteem regulaarsel : NSO; Immunogen järjestus: S7-A71) ja kontrollida proovid lisatakse aukudesse, biotinüülitud avastamis- antikehaga kitse selgesõnalist BAFF-R lisatakse hiljem misjärel vastuvõetud pesemist PBS või TBS puhver. Avidiin-biotiin-peroksidaas Keerukaslisatakse ja seondumata konjugaadid pestakse PBS- või TBS -puhvriga. HRP substraati TMB kasutatakse HRP ensümaatilise vastuse visualiseerimiseks. TMB katalüüsib HRP toota sinist tooni toode, mis lahkus pärast kollaseks koos happeliste stop vastuses . Kollase tihedus on võrdeline plaadile võetud proovi kogusega inimese BAFF-R .
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Märkused
BAFF-R ( lisaks tuntakse Tuumornekroosifaktor väljakutse ülemsugukonda liikme 13C või B-rakkude aktiveeriv väljakutse retseptor) on valk, mis on inimestel on kodeeritud TNFRSF13C geen. Homoloogides BAC klooniga kaardistati BAFFR geen kromosoomile 22q13.1-q13.31. B-raku aktiveeriv väljakutse (BAFF) suurendab B-rakkude ellujäämist in vitro ja on perifeersete B-rakkude elanike regulaator. Selle geeni kodeeritud valk on BAFF-i retseptor ja see on III vormi transmembraanne valk, mis sisaldab ühte rakuvälist fenüülalaniinirikast ruumi . see on arvasin, et see retseptor on peamine retseptor, mis on vajalik BAFF-vahendatud küpsete B-rakkude ellujäämiseks.
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Platvorm
qayee bio human baff r
BAFF-R Antibody |
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| 3578R-100 | Biovision | EUR 316 | |
BAFF-R Antibody |
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| 3578R-30T | Biovision | EUR 146 | |
BIO-X-ACT Short DNA Polymerase |
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| BIO-21065 | Bioline | 500 Units | Ask for price |
BIO-X-ACT Short Mix, 2x |
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| BIO-25026 | Bioline | 500 Reactions | Ask for price |
Polyclonal BAFF-R Antibody |
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| APR15125G | Leading Biology | 0.1mg | EUR 484 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human BAFF-R . This antibody is tested and proven to work in the following applications: |
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BAFF-R Polyclonal Antibody |
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| 3578-100 | Biovision | EUR 316 | |
BAFF-R Blocking Peptide |
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| 3578RBP-50 | Biovision | EUR 153 | |
BAFF-R Recombinant Protein |
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| 90-237 | ProSci | 50 ug | EUR 500.75 |
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Description: BAFF is mainly produced by innate immune cells such as neutrophils, monocytes, macrophages, dendritic cells, follicular dendritic cells. T cells, activated B cells, some malignant B cells and also non-lymphoid cells like astrocytes, synoviocytes and epithelial cells can also produce BAFF. BAFF binds three distinct receptors (BAFF-R, TACI and BCMA) expressed predominantly on B cells, although activated T cells also express BAFF-R. BAFF is a master regulator of peripheral B cell survival, and together with IL-6, promotes Ig class-switching and plasma cell differentiation . Besides its major role in B cell biology, BAFF co-stimulates activated T cells. Deregulated expression of BAFF leads to autoimmune disorders in mice. In humans, elevated levels of soluble BAFF have been detected in the serum of patients with various autoimmune diseases, such as Sjögren’s syndrome , Rheumatoid Arthritis (RA) , Multiple sclerosis (MS) and Systemic Lupus Erythematosus (SLE) . BAFF is also increased levels in some lymphoid cancers. |
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Anti-BAFF-R (human) Monoclonal Antibody (11C1) |
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| M02865 | BosterBio | 100ug | EUR 446 |
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Description: Mouse Monoclonal BAFF-R (human) Antibody (11C1). Validated in Flow Cytometry, IHC and tested in Human. |
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Human B-Cell Activating Factor (BAFF) ELISA Kit |
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| RD-BAFF-Hu-48Tests | Reddot Biotech | 48 Tests | EUR 500 |
Human B-Cell Activating Factor (BAFF) ELISA Kit |
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| RD-BAFF-Hu-96Tests | Reddot Biotech | 96 Tests | EUR 692 |
Human B-Cell Activating Factor (BAFF) ELISA Kit |
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| DLR-BAFF-Hu-48T | DL Develop | 48T | EUR 498 |
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Description: A sandwich quantitative ELISA assay kit for detection of Human B-Cell Activating Factor (BAFF) in samples from serum, plasma, tissue homogenates or other biological fluids. |
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Human B-Cell Activating Factor (BAFF) ELISA Kit |
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| DLR-BAFF-Hu-96T | DL Develop | 96T | EUR 647 |
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Description: A sandwich quantitative ELISA assay kit for detection of Human B-Cell Activating Factor (BAFF) in samples from serum, plasma, tissue homogenates or other biological fluids. |
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Human B-Cell Activating Factor (BAFF) ELISA Kit |
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| RDR-BAFF-Hu-48Tests | Reddot Biotech | 48 Tests | EUR 522 |
Human B-Cell Activating Factor (BAFF) ELISA Kit |
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| RDR-BAFF-Hu-96Tests | Reddot Biotech | 96 Tests | EUR 724 |
BIO |
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| GL6265-1MG | Glentham Life Sciences | 1 mg | EUR 126 |
BIO |
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| GL6265-25MG | Glentham Life Sciences | 25 mg | EUR 447 |
BIO |
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| GL6265-5MG | Glentham Life Sciences | 5 mg | EUR 195 |
BIO |
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| 1673-1 | Biovision | EUR 158 | |
BAFF (B-cell Activating Factor) |
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| RC214-15 | Bio Basic | 5ug | EUR 104.38 |
Mouse B-Cell Activating Factor (BAFF) ELISA Kit |
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| RD-BAFF-Mu-48Tests | Reddot Biotech | 48 Tests | EUR 511 |
Mouse B-Cell Activating Factor (BAFF) ELISA Kit |
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| RD-BAFF-Mu-96Tests | Reddot Biotech | 96 Tests | EUR 709 |
Rat B-Cell Activating Factor (BAFF) ELISA Kit |
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| RD-BAFF-Ra-48Tests | Reddot Biotech | 48 Tests | EUR 534 |
Rat B-Cell Activating Factor (BAFF) ELISA Kit |
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| RD-BAFF-Ra-96Tests | Reddot Biotech | 96 Tests | EUR 742 |
Mouse B-Cell Activating Factor (BAFF) ELISA Kit |
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| DLR-BAFF-Mu-48T | DL Develop | 48T | EUR 508 |
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Description: A sandwich quantitative ELISA assay kit for detection of Mouse B-Cell Activating Factor (BAFF) in samples from serum, plasma, tissue homogenates or other biological fluids. |
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Mouse B-Cell Activating Factor (BAFF) ELISA Kit |
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| DLR-BAFF-Mu-96T | DL Develop | 96T | EUR 661 |
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Description: A sandwich quantitative ELISA assay kit for detection of Mouse B-Cell Activating Factor (BAFF) in samples from serum, plasma, tissue homogenates or other biological fluids. |
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