Polümeeride atmosfäärirõhu plasma plasmajoaga töötlemine võimaldab biomolekulide

Bestmanni-Ohira reaktiiv ja seonduvad diazoühendid asaheterotsüklite sünteesiks

Asaheterotsüklid on üks levinumaid ühendite rühmi, mida leidub arvukates bioaktiivsetes ühendites, looduslikes saadustes ja agrokeemiatoodetes, ja kahtlemata on uued juurdepääsumeetodid neile alati nõudlikud. Saadavate meetodite hulgas on diasoühenditega seotud 1,3-dipolaarsed tsüklilahendusreaktsioonid eriti atraktiivsed, kuna neil on võime regioselektiivselt kiiresti konstrueerida tihedalt funktsionaliseeritud asaheterotsükleid.

Selles kontekstis on Bestmanni-Ohira reaktiivist saanud tuntud reaktiiv 1,3-dipolaarsete tsüklilahendusreaktsioonide korral fosfonüülitud heterotsüklite tekitamiseks, lisaks selle laialdasele kasutamisele homologiseeriva ainena aldehüüdide muundamisel alküünideks.

Selles aruandes kirjeldatakse üksikasjalikult meie jõupingutusi Bestmanni-Ohira reaktiivi ja sellega seotud ühendite sünteetilise kasulikkuse laiendamiseks asaheterotsüklite, näiteks pürasoolide, spirooksindoolide, triasoolide, triasoliinide ja spiropürasoliinide valmistamiseks, rõhutades domino mitmekomponendilisi reaktsioone, kasutades kergesti kättesaadavaid lähteaine reaktiive.

Polümeeride atmosfäärirõhu plasma plasmajoaga töötlemine võimaldab biomolekulide reaktiivivaba kovalentset kinnitamist bioprintimiseks

• 3D-bioprintimine, kus rakke, hüdrogeele ja struktuurpolümeere saab kiht kihi kaupa keerukaks kujundada, lubab meditsiini ja biomeditsiiniteaduste edusamme. Põhimõtteliselt võimaldab see tehnika luua väga patsiendispetsiifilisi haigusmudeleid ja biomeditsiinilisi implantaate. Siiski puudub praegu võimalus trükitud komponentide, näiteks polümeeride ja hüdrogeelide, pinna biosobivuse ja liidese sideme kohandamiseks.

• Siinkohal demonstreerime, et atmosfäärirõhu plasmajoa (APPJ) suudab valkude ja hüdrogeeli reaktiivivaba kovalentse kinnitumise jaoks polümeersed pinnad lokaalselt aktiveerida üheastmelises protsessis soovitud kohtades. Polümeeridena kasutati polüetüleeni ja polü--kaprolaktooni (PCL). Valkude ja hüdrogeeli kovalentset seondumist demonstreeris resistentsus eemaldamise suhtes naatriumdodetsüülsulfaadi (SDS) vary pesemise teel.

• Immobiliseeritud valgu ja hüdrogeeli kihte analüüsiti Fourieri transformatsiooniga infrapuna (FTIR) ja röntgenfotoelektroonspektroskoopia (XPS) abil. Oluline on see, et APPJ pinna aktiveerimine muutis polümeeri pinnad ka kergelt hüdrofiilseteks, nagu on vajalik optimaalseks biosobivuseks.

Veekontaktnurgad olid stabiilsed vahemikus, kus biomolekulide konformatsioon on säilinud. APPJ-de ühe- ja kahekordse elektroodiga kujundusi võrreldi nende omaduste suhtes, mis olid olulised pinna lokaliseerimise funktsionaliseerimise, ploomi pikkuse ja kuju suhtes.

• Efektiivsuse tõendiks bioloogilises kontekstis kasutati rakkude adhesiooni ja proliferatsiooni paranemise tõendamiseks APPJ-ga töödeldud polüetüleeni, mis oli funktsionaliseeritud fibronektiiniga. Nendel tulemustel on oluline mõju 3D-bioprinterite uue põlvkonna väljatöötamisele, mis on võimeline ruumilise mustriga ja kohandatud pinna funktsionaliseerimiseks 3D-printimise käigus in situ.

 

Difluoroisoksasoolatsetofenoon: difluoroalküülimisreaktiiv 1,2-diketoonide orgaaniliste katalüütiliste vinüüloogsete nitroaldoolreaktsioonide jaoks

Difluoroisoksasoolatsetofenoon (DFIO) on välja töötatud uue difluoroalküülimisreagendina, mida saab hõlpsasti valmistada odavatest lähtematerjalidest. DFIO in situ kaugel C-C sidumine annab y, γ-difluoroisoksasoolnitronaadi, mis läbib aluse poolt katalüüsitud vinüüloogse nitroaldooli isatiinidele, bensotiofeen-2,3-dioonile, küllastumata a-ketoesteritele ja tsüklilistele 1,2-diketoonidele.
See orgaanilise katalüütilise debensoaadi vinüülogne nitroaldooli reaktsioon annab uue ja kerge lähenemisviisi erinevate difluoroisoksasooliga asendatud 3-hüdroksü-2-oksindoolide valmistamiseks.

Tampoonide ja reagendi määramise optimeerimine PCR-testimiseks viirusepideemia ajal

Varajane suuremahuline tampooniproovide tegemine on tervishoiuasutustele põhiline vahend viiruse levimuse hindamiseks ja epideemia ajal asjakohaste leevendusmeetmete kehtestamiseks. COVID-19 pandeemia on näidanud, et katsete läbiviimiseks vajaliku keemilise reaktiivi kättesaadavus on sageli pudelikael riigi testimisvõime suurendamisel.

Lisaks jaotub nõudlus ebaühtlaselt rohkem mõjutatud piirkondade (mis vajavad rohkem katseid, mida nad saavad teha) ja vähem mõjutatud piirkondade (millel on vaba tootmisvõimsus) vahel.

Want küsimused viitavad testimisvõimsuse suurendamise võimalusele tampoonide ja reaktiivide optimaalse jaotamise kaudu laboritele. Tõestame, et see on nii, tehes ettepaneku integreeritud programmeerimise koostise kohta, et maksimeerida testide arvu, mida riik saab teha, ja kinnitades meie lähenemisviisi nii Itaalia reaalsete andmete kui ka sünteetiliste juhtumite kohta.

Meie tulemused näitavad, et suurenenud piirkondadevaheline koostöö ja stabiilsem reaktiivide pakkumine (s.t. kohalikest tootmiskohtadest, mitte rahvusvahelistest saadetistest) võib testimisvõimet dramaatiliselt suurendada. Sellest lähtuvalt pakume poliitikakujundajatele ja tervishoiuasutustele lühi- ja pikaajalisi soovitusi.

Reagendivaba kolorimeetriline kolesterooli testriba, mis põhineb nanokeriaali isemoodi värvi muutval omadusel

Kolesterooli mugavaks kolorimeetriliseks määramiseks, ilma et oleks vaja kromogeenset substraati, on välja töötatud paberipõhine testriba, mis koosneb tseeriumoksiidi nanoosakestest (nanotseeria) vesinikperoksiidist (H2O2) sõltuvatest värvi muutvatest nanosüümidest ja kolesterooli oksüdaasist (ChOx). Kolesterooliriba ehitamine algab nanotseeria füüsikalise adsorbeerimisega paberi pinnale, millele järgneb ChOxi kovalentne immobiliseerimine silaniseerimise, kitosaani vahendatud aktiveerimise ja nanotseeriasse sisestatud paberimaatriksite glutaaraldehüüdi töötlemise teel.

Kolesterooli juuresolekul katalüüsib ChOx selle oksüdeerumist, saades H2O2, mis moodustab nanokeeria pinnale peroksiidikompleksi ja kutsub esile nanokeeriasse sisestatud paberi visuaalse värvimuutuse valgest / helekollasest intensiivseks kollaseks / oranžiks, mis määrati mugavalt pilt, mille tavaline nutitelefon on omandanud tarkvaraga ImageJ.

Selle strateegia abil määrati sihtkolesterool konkreetselt tasemeni 40 μM dünaamilise lineaarse kontsentratsioonivahemikuga 0,1–1,5 mM neutraalse pH tingimustes, mis sobib seerumi kolesterooli mõõtmiseks, suurepärase stabiilsusega 20 päeva jooksul ja korduvkasutatavusega, taastades selle algse värvi muutev tegevus Four järjestikuse tsükli jooksul.
Lisaks demonstreeriti selle strateegia praktilist kasulikkust, määrates kolesterooli usaldusväärselt inimese vereseerumi proovides. See uuring demonstreerib ise värvimuutvate nanosüümide potentsiaali kolorimeetrilise pabeririba anduri väljatöötamisel, mis on eriti kasulik seadmeteta hoolduskeskkondades.
Polüsulfureerimine kahepoolse tiamiini disulfureeriva reagendi kaudu

Polüsulfiidi valmistamiseks töötati välja kahepoolse kuueliikmelise tiamiini disulfureeriva reagendi abil efektiivne moduleeriv disulfuratsioon. Ringtüve energia vabanemise kontrolli all tekkisid mitmesugused ebasümmeetrilised trisulfiidid ja tetrasulfiidid nukleofiilide kokkupaneku kaudu väävel-väävel motiivi mõlemale küljele. Sellel strateegial on kõrge efektiivsuse, kergete tingimuste ja üldise ulatusega omadused.

Platelet Derived Growth Factor Receptor Alpha (PDGFRa) Monoclonal Antibody (Human), APC-Cy7
4-CAC060Hu22-APC-Cy7	Cloud-Clone	EUR 715.20 EUR 8148.00 EUR 2154.00 EUR 955.20 EUR 394.80	100ul 10ml 1ml 200ul 20ul
Description: A Mouse monoclonal antibody against Human Platelet Derived Growth Factor Receptor Alpha (PDGFRa). This antibody is labeled with APC-Cy7.

Platelet Derived Growth Factor Receptor Alpha (PDGFRa) Monoclonal Antibody (Human), Biotinylated
4-CAC060Hu22-Biotin	Cloud-Clone	EUR 384.00 EUR 3110.40 EUR 912.00 EUR 472.80 EUR 267.60	100ul 10ml 1ml 200ul 20ul
Description: A Mouse monoclonal antibody against Human Platelet Derived Growth Factor Receptor Alpha (PDGFRa). This antibody is labeled with Biotin.

Platelet Derived Growth Factor Receptor Alpha (PDGFRa) Monoclonal Antibody (Human), Cy3
4-CAC060Hu22-Cy3	Cloud-Clone	EUR 522.00 EUR 5478.00 EUR 1482.00 EUR 682.80 EUR 309.60	100ul 10ml 1ml 200ul 20ul
Description: A Mouse monoclonal antibody against Human Platelet Derived Growth Factor Receptor Alpha (PDGFRa). This antibody is labeled with Cy3.

Platelet Derived Growth Factor Receptor Alpha (PDGFRa) Monoclonal Antibody (Human), FITC
4-CAC060Hu22-FITC	Cloud-Clone	EUR 367.20 EUR 3340.80 EUR 943.20 EUR 463.20 EUR 238.80	100ul 10ml 1ml 200ul 20ul
Description: A Mouse monoclonal antibody against Human Platelet Derived Growth Factor Receptor Alpha (PDGFRa). This antibody is labeled with FITC.

Platelet Derived Growth Factor Receptor Alpha (PDGFRa) Monoclonal Antibody (Human), HRP
4-CAC060Hu22-HRP	Cloud-Clone	EUR 392.40 EUR 3613.20 EUR 1015.20 EUR 495.60 EUR 253.20	100ul 10ml 1ml 200ul 20ul
Description: A Mouse monoclonal antibody against Human Platelet Derived Growth Factor Receptor Alpha (PDGFRa). This antibody is labeled with HRP.

Platelet Derived Growth Factor Receptor Alpha (PDGFRa) Monoclonal Antibody (Human), PE
4-CAC060Hu22-PE	Cloud-Clone	EUR 367.20 EUR 3340.80 EUR 943.20 EUR 463.20 EUR 238.80	100ul 10ml 1ml 200ul 20ul
Description: A Mouse monoclonal antibody against Human Platelet Derived Growth Factor Receptor Alpha (PDGFRa). This antibody is labeled with PE.

Lamin B1 (LMNB1) Polyclonal Antibody (Human, Pig)
4-CAF548Hu01	Cloud-Clone	EUR 280.80 EUR 2774.40 EUR 696.00 EUR 350.40 EUR 249.60	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human, Pig Lamin B1 (LMNB1)

Lamin B1 (LMNB1) Polyclonal Antibody (Human, Pig), APC
4-CAF548Hu01-APC	Cloud-Clone	EUR 391.20 EUR 3606.00 EUR 1014.00 EUR 495.60 EUR 253.20	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human, Pig Lamin B1 (LMNB1). This antibody is labeled with APC.

Lamin B1 (LMNB1) Polyclonal Antibody (Human, Pig), APC-Cy7
4-CAF548Hu01-APC-Cy7	Cloud-Clone	EUR 639.60 EUR 7068.00 EUR 1884.00 EUR 847.20 EUR 362.40	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human, Pig Lamin B1 (LMNB1). This antibody is labeled with APC-Cy7.

Lamin B1 (LMNB1) Polyclonal Antibody (Human, Pig), Biotinylated
4-CAF548Hu01-Biotin	Cloud-Clone	EUR 356.40 EUR 2714.40 EUR 813.60 EUR 433.20 EUR 255.60	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human, Pig Lamin B1 (LMNB1). This antibody is labeled with Biotin.

Lamin B1 (LMNB1) Polyclonal Antibody (Human, Pig), Cy3
4-CAF548Hu01-Cy3	Cloud-Clone	EUR 471.60 EUR 4758.00 EUR 1302.00 EUR 610.80 EUR 288.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human, Pig Lamin B1 (LMNB1). This antibody is labeled with Cy3.

Lamin B1 (LMNB1) Polyclonal Antibody (Human, Pig), FITC
4-CAF548Hu01-FITC	Cloud-Clone	EUR 337.20 EUR 2908.80 EUR 835.20 EUR 420.00 EUR 226.80	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human, Pig Lamin B1 (LMNB1). This antibody is labeled with FITC.

Lamin B1 (LMNB1) Polyclonal Antibody (Human, Pig), HRP
4-CAF548Hu01-HRP	Cloud-Clone	EUR 358.80 EUR 3145.20 EUR 898.80 EUR 448.80 EUR 238.80	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human, Pig Lamin B1 (LMNB1). This antibody is labeled with HRP.

Lamin B1 (LMNB1) Polyclonal Antibody (Human, Pig), PE
4-CAF548Hu01-PE	Cloud-Clone	EUR 337.20 EUR 2908.80 EUR 835.20 EUR 420.00 EUR 226.80	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human, Pig Lamin B1 (LMNB1). This antibody is labeled with PE.

Lamin B1 (LMNB1) Monoclonal Antibody (Human)
4-CAF548Hu22	Cloud-Clone	EUR 290.40 EUR 2932.80 EUR 732.00 EUR 364.80 EUR 254.40	100ul 10ml 1ml 200ul 20ul
Description: A Mouse monoclonal antibody against Human Lamin B1 (LMNB1)

Lamin B1 (LMNB1) Monoclonal Antibody (Human), APC
4-CAF548Hu22-APC	Cloud-Clone	EUR 406.80 EUR 3822.00 EUR 1068.00 EUR 517.20 EUR 259.20	100ul 10ml 1ml 200ul 20ul
Description: A Mouse monoclonal antibody against Human Lamin B1 (LMNB1). This antibody is labeled with APC.

Lamin B1 (LMNB1) Monoclonal Antibody (Human), APC-Cy7
4-CAF548Hu22-APC-Cy7	Cloud-Clone	EUR 669.60 EUR 7500.00 EUR 1992.00 EUR 890.40 EUR 375.60	100ul 10ml 1ml 200ul 20ul
Description: A Mouse monoclonal antibody against Human Lamin B1 (LMNB1). This antibody is labeled with APC-Cy7.

Lamin B1 (LMNB1) Monoclonal Antibody (Human), Biotinylated
4-CAF548Hu22-Biotin	Cloud-Clone	EUR 368.40 EUR 2872.80 EUR 853.20 EUR 448.80 EUR 260.40	100ul 10ml 1ml 200ul 20ul
Description: A Mouse monoclonal antibody against Human Lamin B1 (LMNB1). This antibody is labeled with Biotin.

Lamin B1 (LMNB1) Monoclonal Antibody (Human), Cy3
4-CAF548Hu22-Cy3	Cloud-Clone	EUR 492.00 EUR 5046.00 EUR 1374.00 EUR 639.60 EUR 296.40	100ul 10ml 1ml 200ul 20ul
Description: A Mouse monoclonal antibody against Human Lamin B1 (LMNB1). This antibody is labeled with Cy3.

Lamin B1 (LMNB1) Monoclonal Antibody (Human), FITC
4-CAF548Hu22-FITC	Cloud-Clone	EUR 349.20 EUR 3081.60 EUR 878.40 EUR 436.80 EUR 231.60	100ul 10ml 1ml 200ul 20ul
Description: A Mouse monoclonal antibody against Human Lamin B1 (LMNB1). This antibody is labeled with FITC.

Lamin B1 (LMNB1) Monoclonal Antibody (Human), HRP
4-CAF548Hu22-HRP	Cloud-Clone	EUR 372.00 EUR 3332.40 EUR 945.60 EUR 468.00 EUR 244.80	100ul 10ml 1ml 200ul 20ul
Description: A Mouse monoclonal antibody against Human Lamin B1 (LMNB1). This antibody is labeled with HRP.

Lamin B1 (LMNB1) Monoclonal Antibody (Human), PE
4-CAF548Hu22-PE	Cloud-Clone	EUR 349.20 EUR 3081.60 EUR 878.40 EUR 436.80 EUR 231.60	100ul 10ml 1ml 200ul 20ul
Description: A Mouse monoclonal antibody against Human Lamin B1 (LMNB1). This antibody is labeled with PE.

Lamin A/C (LMNA) Polyclonal Antibody (Human)
4-CAF550Hu01	Cloud-Clone	EUR 285.60 EUR 2853.60 EUR 714.00 EUR 357.60 EUR 252.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Lamin A/C (LMNA)

Lamin A/C (LMNA) Polyclonal Antibody (Human), APC
4-CAF550Hu01-APC	Cloud-Clone	EUR 398.40 EUR 3714.00 EUR 1040.40 EUR 506.40 EUR 256.80	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Lamin A/C (LMNA). This antibody is labeled with APC.

Lamin A/C (LMNA) Polyclonal Antibody (Human), APC-Cy7
4-CAF550Hu01-APC-Cy7	Cloud-Clone	EUR 654.00 EUR 7284.00 EUR 1938.00 EUR 868.80 EUR 369.60	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Lamin A/C (LMNA). This antibody is labeled with APC-Cy7.

Lamin A/C (LMNA) Polyclonal Antibody (Human), Biotinylated
4-CAF550Hu01-Biotin	Cloud-Clone	EUR 362.40 EUR 2793.60 EUR 832.80 EUR 440.40 EUR 258.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Lamin A/C (LMNA). This antibody is labeled with Biotin.

Lamin A/C (LMNA) Polyclonal Antibody (Human), Cy3
4-CAF550Hu01-Cy3	Cloud-Clone	EUR 482.40 EUR 4902.00 EUR 1338.00 EUR 625.20 EUR 291.60	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Lamin A/C (LMNA). This antibody is labeled with Cy3.

Lamin A/C (LMNA) Polyclonal Antibody (Human), FITC
4-CAF550Hu01-FITC	Cloud-Clone	EUR 343.20 EUR 2995.20 EUR 856.80 EUR 428.40 EUR 229.20	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Lamin A/C (LMNA). This antibody is labeled with FITC.

Lamin A/C (LMNA) Polyclonal Antibody (Human), HRP
4-CAF550Hu01-HRP	Cloud-Clone	EUR 366.00 EUR 3238.80 EUR 921.60 EUR 458.40 EUR 242.40	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Lamin A/C (LMNA). This antibody is labeled with HRP.

Lamin A/C (LMNA) Polyclonal Antibody (Human), PE
4-CAF550Hu01-PE	Cloud-Clone	EUR 343.20 EUR 2995.20 EUR 856.80 EUR 428.40 EUR 229.20	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Lamin A/C (LMNA). This antibody is labeled with PE.

Lamin A/C (LMNA) Monoclonal Antibody (Human)
4-CAF550Hu22	Cloud-Clone	EUR 301.20 EUR 3091.20 EUR 768.00 EUR 379.20 EUR 258.00	100ul 10ml 1ml 200ul 20ul
Description: A Mouse monoclonal antibody against Human Lamin A/C (LMNA)

Lamin A/C (LMNA) Monoclonal Antibody (Human), APC
4-CAF550Hu22-APC	Cloud-Clone	EUR 421.20 EUR 4038.00 EUR 1122.00 EUR 538.80 EUR 266.40	100ul 10ml 1ml 200ul 20ul
Description: A Mouse monoclonal antibody against Human Lamin A/C (LMNA). This antibody is labeled with APC.

Lamin A/C (LMNA) Monoclonal Antibody (Human), APC-Cy7
4-CAF550Hu22-APC-Cy7	Cloud-Clone	EUR 699.60 EUR 7932.00 EUR 2100.00 EUR 933.60 EUR 388.80	100ul 10ml 1ml 200ul 20ul
Description: A Mouse monoclonal antibody against Human Lamin A/C (LMNA). This antibody is labeled with APC-Cy7.

Lamin A/C (LMNA) Monoclonal Antibody (Human), Biotinylated
4-CAF550Hu22-Biotin	Cloud-Clone	EUR 379.20 EUR 3031.20 EUR 892.80 EUR 464.40 EUR 265.20	100ul 10ml 1ml 200ul 20ul
Description: A Mouse monoclonal antibody against Human Lamin A/C (LMNA). This antibody is labeled with Biotin.

Lamin A/C (LMNA) Monoclonal Antibody (Human), Cy3
4-CAF550Hu22-Cy3	Cloud-Clone	EUR 512.40 EUR 5334.00 EUR 1446.00 EUR 668.40 EUR 304.80	100ul 10ml 1ml 200ul 20ul
Description: A Mouse monoclonal antibody against Human Lamin A/C (LMNA). This antibody is labeled with Cy3.

Lamin A/C (LMNA) Monoclonal Antibody (Human), FITC
4-CAF550Hu22-FITC	Cloud-Clone	EUR 361.20 EUR 3254.40 EUR 921.60 EUR 454.80 EUR 236.40	100ul 10ml 1ml 200ul 20ul
Description: A Mouse monoclonal antibody against Human Lamin A/C (LMNA). This antibody is labeled with FITC.

Lamin A/C (LMNA) Monoclonal Antibody (Human), HRP
4-CAF550Hu22-HRP	Cloud-Clone	EUR 385.20 EUR 3519.60 EUR 992.40 EUR 486.00 EUR 250.80	100ul 10ml 1ml 200ul 20ul
Description: A Mouse monoclonal antibody against Human Lamin A/C (LMNA). This antibody is labeled with HRP.

Lamin A/C (LMNA) Monoclonal Antibody (Human), PE
4-CAF550Hu22-PE	Cloud-Clone	EUR 361.20 EUR 3254.40 EUR 921.60 EUR 454.80 EUR 236.40	100ul 10ml 1ml 200ul 20ul
Description: A Mouse monoclonal antibody against Human Lamin A/C (LMNA). This antibody is labeled with PE.

ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1) Polyclonal Antibody (Human)
4-CAG369Hu01	Cloud-Clone	EUR 280.80 EUR 2774.40 EUR 696.00 EUR 350.40 EUR 249.60	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1)

ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1) Polyclonal Antibody (Human), APC
4-CAG369Hu01-APC	Cloud-Clone	EUR 391.20 EUR 3606.00 EUR 1014.00 EUR 495.60 EUR 253.20	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1). This antibody is labeled with APC.

ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1) Polyclonal Antibody (Human), APC-Cy7
4-CAG369Hu01-APC-Cy7	Cloud-Clone	EUR 639.60 EUR 7068.00 EUR 1884.00 EUR 847.20 EUR 362.40	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1). This antibody is labeled with APC-Cy7.

ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1) Polyclonal Antibody (Human), Biotinylated
4-CAG369Hu01-Biotin	Cloud-Clone	EUR 356.40 EUR 2714.40 EUR 813.60 EUR 433.20 EUR 255.60	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1). This antibody is labeled with Biotin.

ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1) Polyclonal Antibody (Human), Cy3
4-CAG369Hu01-Cy3	Cloud-Clone	EUR 471.60 EUR 4758.00 EUR 1302.00 EUR 610.80 EUR 288.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1). This antibody is labeled with Cy3.

ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1) Polyclonal Antibody (Human), FITC
4-CAG369Hu01-FITC	Cloud-Clone	EUR 337.20 EUR 2908.80 EUR 835.20 EUR 420.00 EUR 226.80	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1). This antibody is labeled with FITC.

ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1) Polyclonal Antibody (Human), HRP
4-CAG369Hu01-HRP	Cloud-Clone	EUR 358.80 EUR 3145.20 EUR 898.80 EUR 448.80 EUR 238.80	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1). This antibody is labeled with HRP.

ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1) Polyclonal Antibody (Human), PE
4-CAG369Hu01-PE	Cloud-Clone	EUR 337.20 EUR 2908.80 EUR 835.20 EUR 420.00 EUR 226.80	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1). This antibody is labeled with PE.

ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1) Monoclonal Antibody (Human)
4-CAG369Hu22	Cloud-Clone	EUR 310.80 EUR 3249.60 EUR 804.00 EUR 393.60 EUR 262.80	100ul 10ml 1ml 200ul 20ul
Description: A Mouse monoclonal antibody against Human ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1)

ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1) Monoclonal Antibody (Human), APC
4-CAG369Hu22-APC	Cloud-Clone	EUR 436.80 EUR 4254.00 EUR 1176.00 EUR 560.40 EUR 272.40	100ul 10ml 1ml 200ul 20ul
Description: A Mouse monoclonal antibody against Human ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1). This antibody is labeled with APC.

ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1) Monoclonal Antibody (Human), APC-Cy7
4-CAG369Hu22-APC-Cy7	Cloud-Clone	EUR 729.60 EUR 8364.00 EUR 2208.00 EUR 976.80 EUR 402.00	100ul 10ml 1ml 200ul 20ul
Description: A Mouse monoclonal antibody against Human ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1). This antibody is labeled with APC-Cy7.

ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1) Monoclonal Antibody (Human), Biotinylated
4-CAG369Hu22-Biotin	Cloud-Clone	EUR 390.00 EUR 3189.60 EUR 932.40 EUR 480.00 EUR 270.00	100ul 10ml 1ml 200ul 20ul
Description: A Mouse monoclonal antibody against Human ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1). This antibody is labeled with Biotin.

ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1) Monoclonal Antibody (Human), Cy3
4-CAG369Hu22-Cy3	Cloud-Clone	EUR 532.80 EUR 5622.00 EUR 1518.00 EUR 697.20 EUR 313.20	100ul 10ml 1ml 200ul 20ul
Description: A Mouse monoclonal antibody against Human ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1). This antibody is labeled with Cy3.

ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1) Monoclonal Antibody (Human), FITC
4-CAG369Hu22-FITC	Cloud-Clone	EUR 373.20 EUR 3427.20 EUR 964.80 EUR 471.60 EUR 242.40	100ul 10ml 1ml 200ul 20ul
Description: A Mouse monoclonal antibody against Human ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1). This antibody is labeled with FITC.

ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1) Monoclonal Antibody (Human), HRP
4-CAG369Hu22-HRP	Cloud-Clone	EUR 398.40 EUR 3706.80 EUR 1039.20 EUR 505.20 EUR 255.60	100ul 10ml 1ml 200ul 20ul
Description: A Mouse monoclonal antibody against Human ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1). This antibody is labeled with HRP.

ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1) Monoclonal Antibody (Human), PE
4-CAG369Hu22-PE	Cloud-Clone	EUR 373.20 EUR 3427.20 EUR 964.80 EUR 471.60 EUR 242.40	100ul 10ml 1ml 200ul 20ul
Description: A Mouse monoclonal antibody against Human ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1). This antibody is labeled with PE.

Voltage Dependent Anion Channel Protein 1 (VDAC1) Polyclonal Antibody (Human)
4-CAG901Hu01	Cloud-Clone	EUR 289.20 EUR 2900.40 EUR 724.80 EUR 361.20 EUR 253.20	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Voltage Dependent Anion Channel Protein 1 (VDAC1)

Voltage Dependent Anion Channel Protein 1 (VDAC1) Polyclonal Antibody (Human), APC
4-CAG901Hu01-APC	Cloud-Clone	EUR 403.20 EUR 3778.80 EUR 1057.20 EUR 512.40 EUR 258.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Voltage Dependent Anion Channel Protein 1 (VDAC1). This antibody is labeled with APC.

Voltage Dependent Anion Channel Protein 1 (VDAC1) Polyclonal Antibody (Human), APC-Cy7
4-CAG901Hu01-APC-Cy7	Cloud-Clone	EUR 663.60 EUR 7413.60 EUR 1970.40 EUR 880.80 EUR 373.20	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Voltage Dependent Anion Channel Protein 1 (VDAC1). This antibody is labeled with APC-Cy7.

Voltage Dependent Anion Channel Protein 1 (VDAC1) Polyclonal Antibody (Human), Biotinylated
4-CAG901Hu01-Biotin	Cloud-Clone	EUR 366.00 EUR 2840.40 EUR 844.80 EUR 445.20 EUR 259.20	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Voltage Dependent Anion Channel Protein 1 (VDAC1). This antibody is labeled with Biotin.

Voltage Dependent Anion Channel Protein 1 (VDAC1) Polyclonal Antibody (Human), Cy3
4-CAG901Hu01-Cy3	Cloud-Clone	EUR 488.40 EUR 4988.40 EUR 1359.60 EUR 633.60 EUR 295.20	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Voltage Dependent Anion Channel Protein 1 (VDAC1). This antibody is labeled with Cy3.

Voltage Dependent Anion Channel Protein 1 (VDAC1) Polyclonal Antibody (Human), FITC
4-CAG901Hu01-FITC	Cloud-Clone	EUR 346.80 EUR 3046.80 EUR 868.80 EUR 433.20 EUR 230.40	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Voltage Dependent Anion Channel Protein 1 (VDAC1). This antibody is labeled with FITC.

Voltage Dependent Anion Channel Protein 1 (VDAC1) Polyclonal Antibody (Human), HRP
4-CAG901Hu01-HRP	Cloud-Clone	EUR 369.60 EUR 3294.00 EUR 936.00 EUR 464.40 EUR 243.60	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Voltage Dependent Anion Channel Protein 1 (VDAC1). This antibody is labeled with HRP.

Voltage Dependent Anion Channel Protein 1 (VDAC1) Polyclonal Antibody (Human), PE
4-CAG901Hu01-PE	Cloud-Clone	EUR 346.80 EUR 3046.80 EUR 868.80 EUR 433.20 EUR 230.40	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Voltage Dependent Anion Channel Protein 1 (VDAC1). This antibody is labeled with PE.

Voltage Dependent Anion Channel Protein 1 (VDAC1) Monoclonal Antibody (Human)
4-CAG901Hu22	Cloud-Clone	EUR 310.80 EUR 3249.60 EUR 804.00 EUR 393.60 EUR 262.80	100ul 10ml 1ml 200ul 20ul
Description: A Mouse monoclonal antibody against Human Voltage Dependent Anion Channel Protein 1 (VDAC1)

Voltage Dependent Anion Channel Protein 1 (VDAC1) Monoclonal Antibody (Human), APC
4-CAG901Hu22-APC	Cloud-Clone	EUR 436.80 EUR 4254.00 EUR 1176.00 EUR 560.40 EUR 272.40	100ul 10ml 1ml 200ul 20ul
Description: A Mouse monoclonal antibody against Human Voltage Dependent Anion Channel Protein 1 (VDAC1). This antibody is labeled with APC.

Voltage Dependent Anion Channel Protein 1 (VDAC1) Monoclonal Antibody (Human), APC-Cy7
4-CAG901Hu22-APC-Cy7	Cloud-Clone	EUR 729.60 EUR 8364.00 EUR 2208.00 EUR 976.80 EUR 402.00	100ul 10ml 1ml 200ul 20ul
Description: A Mouse monoclonal antibody against Human Voltage Dependent Anion Channel Protein 1 (VDAC1). This antibody is labeled with APC-Cy7.

Voltage Dependent Anion Channel Protein 1 (VDAC1) Monoclonal Antibody (Human), Biotinylated
4-CAG901Hu22-Biotin	Cloud-Clone	EUR 390.00 EUR 3189.60 EUR 932.40 EUR 480.00 EUR 270.00	100ul 10ml 1ml 200ul 20ul
Description: A Mouse monoclonal antibody against Human Voltage Dependent Anion Channel Protein 1 (VDAC1). This antibody is labeled with Biotin.

Voltage Dependent Anion Channel Protein 1 (VDAC1) Monoclonal Antibody (Human), Cy3
4-CAG901Hu22-Cy3	Cloud-Clone	EUR 532.80 EUR 5622.00 EUR 1518.00 EUR 697.20 EUR 313.20	100ul 10ml 1ml 200ul 20ul
Description: A Mouse monoclonal antibody against Human Voltage Dependent Anion Channel Protein 1 (VDAC1). This antibody is labeled with Cy3.

Voltage Dependent Anion Channel Protein 1 (VDAC1) Monoclonal Antibody (Human), FITC
4-CAG901Hu22-FITC	Cloud-Clone	EUR 373.20 EUR 3427.20 EUR 964.80 EUR 471.60 EUR 242.40	100ul 10ml 1ml 200ul 20ul
Description: A Mouse monoclonal antibody against Human Voltage Dependent Anion Channel Protein 1 (VDAC1). This antibody is labeled with FITC.

Voltage Dependent Anion Channel Protein 1 (VDAC1) Monoclonal Antibody (Human), HRP
4-CAG901Hu22-HRP	Cloud-Clone	EUR 398.40 EUR 3706.80 EUR 1039.20 EUR 505.20 EUR 255.60	100ul 10ml 1ml 200ul 20ul
Description: A Mouse monoclonal antibody against Human Voltage Dependent Anion Channel Protein 1 (VDAC1). This antibody is labeled with HRP.

Voltage Dependent Anion Channel Protein 1 (VDAC1) Monoclonal Antibody (Human), PE
4-CAG901Hu22-PE	Cloud-Clone	EUR 373.20 EUR 3427.20 EUR 964.80 EUR 471.60 EUR 242.40	100ul 10ml 1ml 200ul 20ul
Description: A Mouse monoclonal antibody against Human Voltage Dependent Anion Channel Protein 1 (VDAC1). This antibody is labeled with PE.

Human Angiotensin II (AngII) CLIA Kit
4-CCA005Hu	Cloud-Clone	EUR 6837.60 EUR 3673.20 EUR 896.40	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Description: Competitive Inhibition chemiluminescent immunoassay for detection of Human Angiotensin II (AngII)Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Human Glycated Hemoglobin A1c (HbA1c) CLIA Kit
4-CCA190Hu	Cloud-Clone	EUR 6178.80 EUR 3324.00 EUR 819.60	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Description: Competitive Inhibition chemiluminescent immunoassay for detection of Human Glycated Hemoglobin A1c (HbA1c)serum, plasma and erythrocyte lysates

Human Anti-Mullerian Hormone (AMH) CLIA Kit
4-CCA228Hu	Cloud-Clone	EUR 6837.60 EUR 3673.20 EUR 896.40	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Description: Competitive Inhibition chemiluminescent immunoassay for detection of Human Anti-Mullerian Hormone (AMH)Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

General Bovine Serum Albumin (BSA) CLIA Kit
4-CCA248Ge	Cloud-Clone	EUR 6649.20 EUR 3573.60 EUR 874.80	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Description: Competitive Inhibition chemiluminescent immunoassay for detection of General Bovine Serum Albumin (BSA)Serum, plasma and other biological fluids

Cattle Substance P (SP) CLIA Kit
4-CCA393Bo	Cloud-Clone	EUR 8157.60 EUR 4371.60 EUR 1052.40	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Description: Competitive Inhibition chemiluminescent immunoassay for detection of Cattle Substance P (SP)Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Dog Substance P (SP) CLIA Kit
4-CCA393Ca	Cloud-Clone	EUR 7780.80 EUR 4172.40 EUR 1008.00	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Description: Competitive Inhibition chemiluminescent immunoassay for detection of Dog Substance P (SP)Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Guinea pig Substance P (SP) CLIA Kit
4-CCA393Gu	Cloud-Clone	EUR 7780.80 EUR 4172.40 EUR 1008.00	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Description: Competitive Inhibition chemiluminescent immunoassay for detection of Guinea pig Substance P (SP)Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Human Substance P (SP) CLIA Kit
4-CCA393Hu	Cloud-Clone	EUR 6837.60 EUR 3673.20 EUR 896.40	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Description: Competitive Inhibition chemiluminescent immunoassay for detection of Human Substance P (SP)Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mouse Substance P (SP) CLIA Kit
4-CCA393Mu	Cloud-Clone	EUR 7026.00 EUR 3772.80 EUR 919.20	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Description: Competitive Inhibition chemiluminescent immunoassay for detection of Mouse Substance P (SP)Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Rat Substance P (SP) CLIA Kit
4-CCA393Ra	Cloud-Clone	EUR 7404.00 EUR 3973.20 EUR 963.60	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Description: Competitive Inhibition chemiluminescent immunoassay for detection of Rat Substance P (SP)serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Parandatud HPLC tingimused eumelaniini ja feomelaniini sisalduse määramiseks bioloogilistes proovides, kasutades ioonpaarreagenti

Eumelaniini ja feomelaniini leeliseline vesinikperoksiidi oksüdeerimine (AHPO), kaks peamist melaniinipigmentide klassi, annavad pürrool-2,3,5-trikarboksüülhappe (PTCA), pürrool-2,3-dikarboksüülhappe (PDCA) ja pürrool-2, 3,4,5-tetrakarboksüülhape (PTeCA) eumelaniinist ja tiasool-2,4,5-trikarboksüülhape (TTCA) ja tiasool-4,5-dikarboksüülhape (TDCA) feomelaniinist.
Nende viie markeri kvantifitseerimine HPLC abil annab kasulikku teavet melaniinide koguse ja struktuurilise mitmekesisuse kohta erinevates bioloogilistes proovides.

Nende markerite HPLC analüüsil, kasutades algupärast meetodit 0,1 M kaaliumfosfaatpuhvrit (pH 2,1): metanool = 99: 1 (PTeCA jaoks 85:15) pöördfaasilises kolonnis, oli mõningaid probleeme, sealhulgas kolonni lühike eluiga ja , välja arvatud peamine eumelaniini marker PTCA, kattusid teised markerid aeg-ajalt häirivate piikidega proovides, mis sisaldasid ainult nende markerite jälgi.
Nendest probleemidest saab üle, lisades ioonide paarireagendi anioonide jaoks, näiteks tetra-n-butüülammooniumbromiid (1 mM), pidurdamaks di-, tri- ja tetra-karboksüülhapete elueerimist. Metanooli kontsentratsiooni suurendati 17% -ni (PTeCA puhul 30% -ni) ning markerite lineaarsus, reprodutseeritavus ja taastumine selle täiustatud meetodiga on hea kuni suurepärane.
Seda täiustatud HPLC meetodit võrreldi algse meetodiga, kasutades sünteetilisi melaniine, hiirekarva, inimese juukseid ja inimese epidermise proove. Lisaks PTCA-le näitas feomelaniini peamine marker TTCA suurepäraseid korrelatsioone mõlema HPLC meetodi vahel.

Teised markerid näitasid täiustatud meetodiga häirivate piikide nõrgenemist. Soovitame seda täiustatud HPLC meetodit AHPO-le järgnevate melaniinimarkerite kvantitatiivseks analüüsiks selle lihtsuse, täpsuse ja reprodutseeritavuse tõttu.

Tetrasiinist saadud infrapuna-lähedane värv kui hõlbus reaktiiv sihipäraste fotoakustiliste pildiagentide väljatöötamiseks

Sihtotstarbeliste fotoakustiliste pildistusvahendite loomiseks töötati välja lihtne fotoakustiline värvaine kui lihtne kasutada reaktiiv. Pliimolekul valmistati tõhusa kaheastmelise sünteesi teel odavast kaubanduslikult saadaval olevast lähtematerjalist. Värvaine kaasasündinud albumiiniga seonduvate omaduste tõttu on saadud tetrasiinist saadud värv võimeline kasvajas lokaliseeruma ja selle bioloogiline poolestusaeg on paar tundi, mis võimaldab optimeeritud jaotumisprofiili.
Tetrasiini olemasolu võimaldab omakorda siduda albumiini siduva optoakustilise signaalivahendi laia sihtmärgiks olevate molekulidega. Platvormi kasulikkuse ja kasutusmugavuse demonstreerimiseks loodi uus fotoakustiline proov kaltsiumi akretsiooni kuvamiseks, kasutades üheastmelist bioortogonaalset sidestusreaktsiooni, kus hiirtel saadi põlveliigese kõrge eraldusvõimega fotoakustilised kujutised juba üks tund pärast süstimine. Seejärel määrati kogu keha jaotus märgistades sondi 99mTc-ga ja tehes kudede loendamise pärast lahkamist.
Want uuringud koos kasvajakujutiste ja in vitro albumiiniga seondumise uuringutega näitasid, et südamiku fotoakustilist kontrastainet saab kujutada in vivo ja seda saab hõlpsasti seostada sihtmolekulidega elundispetsiifilise omastamise jaoks.