Toote ülevaade
Kortikosterooni ELISA seadmed on agressiivne ensüümiga seotud immunosorbentanalüüs, mis on ette nähtud kortikosterooni vahemike kvantitatiivseks mõõtmiseks plasmas, seerumis, uriinis, piimas, süljes ja raku traditsioonilises supernatandis.
Kortikosterooni spetsiifiline antikeha on eelnevalt kaetud 96-augulistele plaatidele ja blokeeritud. Nõuded või proovide vaatamine lisatakse süvenditesse ja seejärel lisatakse biotinüülitud kortikosteroon, mis võetakse vastu pesupuhvriga pestes. Lisatakse Avidin-Biotin-Peroxidase Advanced ja seondumata konjugaadid pestakse pesupuhvriga. Seejärel kasutatakse TMB -d HRP ensümaatilise vastuse visualiseerimiseks. TMP katalüüsib HRP, et saada sinine värvitoon, mis muutub pärast happelise lakkamise lisamist kollaseks. Kollase värvuse tihedus on pöördvõrdeline plaadile kogunenud kortikosterooni kogusega.
Peamised võimalused ja üksikasjad
- Tundlikkus: 0. Kolm ng/ml
- Erinevad: 0,391 ng/ml – 100 ng/ml
- Mustri sort: rakutraditsiooni supernatant, piim, plasma, sülg, seerum, uriin
- Tuvastamismetoodika: kolorimeetriline
- Analüüsi tüüp: agressiivne
- Reageerib järgmiste inimestega: hiir, rott, inimene, paavian
Toote üksikasjad
| Tundlikkus: | 27. Null pg/ml (varieerub 32–20 000 pg/ml) |
| Analüüsi aeg: | Kolm tundi |
| Eesmärgid: | ELISA, kolorimeetriline tuvastus |
| Tarkvara märkused: | Kortikosterooni kvantitatiivse tahtejõu jaoks traditsioonilistes supernatantides, väljaheites, plasmas, seerumis ja mis tahes liigi süljes. Viidatud mustrid sisaldavad rakulüsaati, uriini ja täis verd. |
| Lainepikkus: | 405 nm |
| Liigi reaktsioonivõime: | Liigid erapooletud |
| Ristreaktsioon: | Kortikosteroon (100%), desoksükortikosteroon (21,3%), desoksükortikosteroon (21,0%), progesteroon (0,46%), testosteroon (0,31%), tetrahüdrokortikosteroon (0,28%), aldosteroon (0,18%), kortisool (0,046%) ja <0,03 %: Pregnenoloon, östradiool, kortisoon, 11-dehüdrokortikosteroonatsetaat |
| Kohaletoimetamine: | Sinine jää pole külmunud |
| Pikaajaline ladustamine: | +4 ° C |
| Sisu: | DxS IgG mikrotiiterplaat, analüüsipuhvri 15 fookus, steroidide nihke reagent, konjugaat, antikeha, pesupuhvri fookus, tavaline, pNpp substraat, katkestuse eraldusvõime |
| Teaduslik taust: | Kortikosteroon on glükokortikoid, mida eritab neerupealise koor. Kortikosterooni toodetakse vastuseks adrenokortikotroopse hormooni (ACTH) neerupealise koore stimuleerimisele ja see on aldosterooni eelkäija. On tõestatud, et kortikosterooni tootmine laieneb stressiga, kusjuures kõrgenenud kortikosterooni vahemikud on seotud pikaajalise mälu taastamise ja põletuskahjustuste kahjustusega. Koos stressivahemikega arvatakse, et kortikosteroon mängib une-ärkveloleku mustrites otsustavat funktsiooni. |
| Tehnilised andmed/toote märkused: | Viidatud proovid: Hormoonide ELISA -d: Viidatud proovid Metsloomade endokrinoloogia: Viidatud mustrid |
| Protokoll: | Kiire protokoll seerumi/plasmaproovide väikeste koguste jaoks
Lõplik lahjendus on ~ 1: 40 (see järelejäänud lahjendus ei ole iga mustri puhul vastuvõetav; seega peaks optimaalse lahjenduse otsustama iga viimistlustarbija oma katsemustrirühma kohta.) |
| Reguleeriv alaline seis: | RUO – analüüsi kasutamine ainult |
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Description: A competitive ELISA for quantitative measurement of Porcine Rheumatoid arthritis associated nuclear artigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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| E04R0342-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A competitive ELISA for quantitative measurement of Rabbit Rheumatoid arthritis associated nuclear artigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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