corticosterone

Kortikosterooni ELISA komplekt

Toote ülevaade

Kortikosterooni ELISA seadmed on agressiivne ensüümiga seotud immunosorbentanalüüs, mis on ette nähtud kortikosterooni vahemike kvantitatiivseks mõõtmiseks plasmas, seerumis, uriinis, piimas, süljes ja raku traditsioonilises supernatandis.

Kortikosterooni spetsiifiline antikeha on eelnevalt kaetud 96-augulistele plaatidele ja blokeeritud. Nõuded või proovide vaatamine lisatakse süvenditesse ja seejärel lisatakse biotinüülitud kortikosteroon, mis võetakse vastu pesupuhvriga pestes. Lisatakse Avidin-Biotin-Peroxidase Advanced ja seondumata konjugaadid pestakse pesupuhvriga. Seejärel kasutatakse TMB -d HRP ensümaatilise vastuse visualiseerimiseks. TMP katalüüsib HRP, et saada sinine värvitoon, mis muutub pärast happelise lakkamise lisamist kollaseks. Kollase värvuse tihedus on pöördvõrdeline plaadile kogunenud kortikosterooni kogusega.

Peamised võimalused ja üksikasjad

  • Tundlikkus: 0. Kolm ng/ml
  • Erinevad: 0,391 ng/ml – 100 ng/ml
  • Mustri sort: rakutraditsiooni supernatant, piim, plasma, sülg, seerum, uriin
  • Tuvastamismetoodika: kolorimeetriline
  • Analüüsi tüüp: agressiivne
  • Reageerib järgmiste inimestega: hiir, rott, inimene, paavian
corticosterone
corticosterone

Toote üksikasjad

Tundlikkus: 27. Null pg/ml (varieerub 32–20 000 pg/ml)
Analüüsi aeg: Kolm tundi
Eesmärgid: ELISA, kolorimeetriline tuvastus
Tarkvara märkused: Kortikosterooni kvantitatiivse tahtejõu jaoks traditsioonilistes supernatantides, väljaheites, plasmas, seerumis ja mis tahes liigi süljes. Viidatud mustrid sisaldavad rakulüsaati, uriini ja täis verd.
Lainepikkus: 405 nm
Liigi reaktsioonivõime: Liigid erapooletud
Ristreaktsioon: Kortikosteroon (100%), desoksükortikosteroon (21,3%), desoksükortikosteroon (21,0%), progesteroon (0,46%), testosteroon (0,31%), tetrahüdrokortikosteroon (0,28%), aldosteroon (0,18%), kortisool (0,046%) ja <0,03 %: Pregnenoloon, östradiool, kortisoon, 11-dehüdrokortikosteroonatsetaat
Kohaletoimetamine: Sinine jää pole külmunud
Pikaajaline ladustamine: +4 ° C
Sisu: DxS IgG mikrotiiterplaat, analüüsipuhvri 15 fookus, steroidide nihke reagent, konjugaat, antikeha, pesupuhvri fookus, tavaline, pNpp substraat, katkestuse eraldusvõime
Teaduslik taust: Kortikosteroon on glükokortikoid, mida eritab neerupealise koor. Kortikosterooni toodetakse vastuseks adrenokortikotroopse hormooni (ACTH) neerupealise koore stimuleerimisele ja see on aldosterooni eelkäija. On tõestatud, et kortikosterooni tootmine laieneb stressiga, kusjuures kõrgenenud kortikosterooni vahemikud on seotud pikaajalise mälu taastamise ja põletuskahjustuste kahjustusega. Koos stressivahemikega arvatakse, et kortikosteroon mängib une-ärkveloleku mustrites otsustavat funktsiooni.
Tehnilised andmed/toote märkused: Viidatud proovid:
Hormoonide ELISA -d: Viidatud proovid
Metsloomade endokrinoloogia: Viidatud mustrid
Protokoll: Kiire protokoll seerumi/plasmaproovide väikeste koguste jaoks 

  1. Laske kõigil valikutel enne kasutamist toatemperatuurini jõuda.
  2. Alikvootige 10 µl igast mustrist mikrofuugi tuubi.
  3. Tehke 1 ml 1: 100 steroidide nihkereagendi (SDR) eraldusvõime (koheselt enne kasutamist) deioniseeritud vees või PBS -is (mitte analüüsipuhvris)
  4. Lisage igale mustritorule 10 µl 1: 100 SDR -i.
  5. Vortex, lase seista> 5 minutit varem kui EIA puhvriga lahjendamine.
  6. Lisage 380 µl EIA analüüsipuhvrit igasse plastaklaasi, pöörake.

Lõplik lahjendus on ~ 1: 40  (see järelejäänud lahjendus ei ole iga mustri puhul vastuvõetav; seega peaks optimaalse lahjenduse otsustama iga viimistlustarbija oma katsemustrirühma kohta.)

Reguleeriv alaline seis: RUO – analüüsi kasutamine ainult

 

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Caprine arthritis-encephalitis virus RT PCR kit

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Caprine arthritis-encephalitis virus RT PCR kit

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  • Contact us in order to know the reactivity of the kit.
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Caprine arthritis-encephalitis virus One-Step PCR kit

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  • Contact us in order to know the reactivity of the kit.
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Caprine arthritis-encephalitis virus.

Caprine arthritis-encephalitis virus One-Step PCR kit

Oneq-V320-150R 150T
EUR 1060.6
  • Contact us in order to know the reactivity of the kit.
Description: Real Time PCR Kit is a screening assay for a rapid and accurate detection of Caprine arthritis-encephalitis virus.

Caprine arthritis-encephalitis virus One-Step PCR kit

Oneq-V320-50R 50T
EUR 751.75
  • Contact us in order to know the reactivity of the kit.
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Mouse Rheumatoid arthritis associated nuclear artigen ELISA kit

E03R0342-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Mouse Rheumatoid arthritis associated nuclear artigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Rheumatoid arthritis associated nuclear artigen ELISA kit

E03R0342-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Mouse Rheumatoid arthritis associated nuclear artigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Rheumatoid arthritis associated nuclear artigen ELISA kit

E03R0342-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Mouse Rheumatoid arthritis associated nuclear artigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Rheumatoid arthritis associated nuclear artigen ELISA kit

E02R0342-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rat Rheumatoid arthritis associated nuclear artigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Rheumatoid arthritis associated nuclear artigen ELISA kit

E02R0342-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rat Rheumatoid arthritis associated nuclear artigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Rheumatoid arthritis associated nuclear artigen ELISA kit

E02R0342-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rat Rheumatoid arthritis associated nuclear artigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Rheumatoid arthritis associated nuclear artigen ELISA kit

E01R0342-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Human Rheumatoid arthritis associated nuclear artigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Rheumatoid arthritis associated nuclear artigen ELISA kit

E01R0342-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Human Rheumatoid arthritis associated nuclear artigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Rheumatoid arthritis associated nuclear artigen ELISA kit

E01R0342-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Human Rheumatoid arthritis associated nuclear artigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Rheumatoid arthritis associated nuclear artigen ELISA kit

E04R0342-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rabbit Rheumatoid arthritis associated nuclear artigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Rheumatoid arthritis associated nuclear artigen ELISA kit

E04R0342-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rabbit Rheumatoid arthritis associated nuclear artigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Rheumatoid arthritis associated nuclear artigen ELISA kit

E04R0342-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rabbit Rheumatoid arthritis associated nuclear artigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Rheumatoid arthritis associated nuclear artigen ELISA kit

E06R0342-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Goat Rheumatoid arthritis associated nuclear artigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Rheumatoid arthritis associated nuclear artigen ELISA kit

E06R0342-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Goat Rheumatoid arthritis associated nuclear artigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Rheumatoid arthritis associated nuclear artigen ELISA kit

E06R0342-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Goat Rheumatoid arthritis associated nuclear artigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Rheumatoid arthritis associated nuclear artigen ELISA kit

E07R0342-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Porcine Rheumatoid arthritis associated nuclear artigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Rheumatoid arthritis associated nuclear artigen ELISA kit

E07R0342-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Porcine Rheumatoid arthritis associated nuclear artigen in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

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