Kontraŭhoma IgG Fc Monoklona Malĉefa Antikorpo (Min X Ms, Rt, Rb, Ch, Gt)
Priskribo
Ĝenerale, kapraj kontraŭhomaj aŭ azenaj kontraŭhomaj IgG-poliklonaj antikorpoj estas uzataj kiel la duaranga antikorpo por detekti homajn primarajn antikorpojn. Tamen, ĉar plurklonaj antikorpoj povas rekoni multajn epitopojn, la fono kaj la kruca reagemo en la provoj estas kutime altaj, kio malfaciligas la rezultojn interpreti. Por minimumigi la fonon kaj transversan reagemon kaj plibonigi mult -al-multan konsistencon, unikan GenScript Mouse Anti-Human IgG Fc Antibody (50B4A9) [HRP], mAb estis disvolvita por esti uzata kiel duaranga antikorpo. La supereco de ĉi tiu antikorpo estas, ke ĝi povas esti uzata kiel idiotipa antikorpo por detekti drogan metabolon in vivo.
Gastigantaj Specioj
Muso
Antigena Specio
Homa
Konjugacio
Peroksidazo (Kreno)
Immunogen
Homa IgG (H&L)
Purigo
Proteina A-afineca kolono
Laboraj koncentriĝoj por specifaj aplikoj devas esti determinitaj de la enketisto. La taŭgaj koncentriĝoj povas esti trafitaj de primara antikorpa afineco, antigena koncentriĝo, la sentemo de la metodo de detekto, temperaturo, la longo de la inkubacioj kaj aliaj faktoroj. La taŭgeco de ĉi tiu antikorpo por aplikoj krom tiuj listigitaj sube ne estis determinita. La jenaj koncentriĝaj gamoj rekomendas komencajn punktojn por ĉi tiu produkto.
Kontraŭ-NHS antikorpoj estas ofertitaj de kelkaj provizantoj. Ĉi tiu cela geno kodas la proteinon “NHS-aktina remodela reguligilo” ĉe homoj kaj ankaŭ povas esti konata kiel CTRCT40, CXN, SCML1, sindromo de Nance-Horan kaj sindromo de Nance-Horan (denaskaj akvofaloj kaj dentaj anomalioj). Strukture, la proteino laŭdire havas 179,1 kilodaltonojn en maso. Surbaze de gennomo, hundaj, porkaj, simiaj, musaj kaj rataj ortologoj ankaŭ troveblas. Por pli ampleksaj informoj pri antikorpaj produktoj (kiel imunogen, specifeco, aplikoj kaj pli), vizitu la paĝon de la provizanto.
hns, Polyclonal Antibody
Abstrakta
Histon-simila nukleida struktura proteino (H-NS) estas modula proteino, kiu estas asociita kun la bakteria nukleido. Ni uzis kromatin-imunoprecipiton por determini la ligajn lokojn de H-NS kaj RNA-polimerazo sur la Salmonella enterica serovar-Typhimurium-kromosomo. Ni trovis, ke H-NS ne ligas al aktive transskribitaj genoj kaj ne kunlokiĝas kun RNA-polimerazo. Ĉi tio montras, ke H-NS ĉefe silentigas genan esprimon limigante la aliron de RNA-polimerazo al la DNA. H-NS antaŭe estis montrita preferate ligi al kurba DNA in vitro. Fakte, je la genomika nivelo ni malkovris, ke la nivelo de ligado de H-NS pli bone rilatas al la AT-enhavo de DNA. Ĉi tio probable havas evoluajn konsekvencojn, ĉar ni montras, ke H-NS ligas al multaj Salmoneloj genoj akiritaj per flanka transdono de genoj, kaj funkcias kiel gena dampilo. La forigo de H-NS el la ĉelo kaŭzas nekontrolitan esprimon de pluraj insuloj de Salmonella patogeneco, kaj ni montras, ke ĉi tio havas malutilajn konsekvencojn por bakteria taŭgeco. Nia malkovro de ĉi tiu nova rolo por H-NS povas havi implicojn por la akiro de fremdaj genoj de enaj bakterioj .
Sinoptiko
En la lastaj jardekoj, gena silentigo estis bone karakterizita en plantoj kaj bestoj, kaj implikas la preventon de transskribo per DNA-metiligo kaj histono-modifo, aŭ enmiksiĝo kun traduko de malgrandaj RNA-molekuloj. Ĉi tiu numero de PLoS-Patogenoj raportas la malkovron, ke tutmonda gena silentigo okazas ankaŭ en bakterioj. La nova me mechanismanismo estas perata de la tre abunda histona-simila nukleida struktura proteino (H-NS), kiu blokas la esprimon de 254 genoj en sovaĝa tipo Salmonella.. Multaj el ĉi tiuj genoj estis akiritaj per horizontala transdono de genoj, inkluzive de insuloj de patogeneco, kaj ĉi tiuj estas silentigitaj per la ligo de H-NS al AT-riĉaj kromosomaj regionoj. La studo malkaŝas, ke H-NS malhelpas la nekontrolitan transskribon de genoj ene de patogenaj insuloj por certigi, ke bakteria taŭgeco estas konservata. Estas sugestite ke H-NS ludas rolon en bakteria evoluo influante kaj la akiron kaj prizorgadon de fremda DNA.
anti- Antibody^Polyclonal antibody control antibody
Description: ARHGDIA regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them.
Description: ARHGDIA regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them.
Description: The CLCN5 gene encodes the chloride channel Cl-/H+ exchanger ClC-5. This gene encodes a member of the ClC family of chloride ion channels and ion transporters. The encoded protein is primarily localized to endosomal membranes and may function to facilitate albumin uptake by the renal proximal tubule. Mutations in this gene have been found in Dent disease and renal tubular disorders complicated by nephrolithiasis. Alternatively spliced transcript variants have been found for this gene.
Description: This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced.
Description: This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19.
Description: This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis and the maintenance of cellular morphology. This gene encodes a protein that can form homo- and heterooligomeric filaments, and may contribute to the formation of neurofibrillary tangles in Alzheimer's disease. Alternatively spliced transcript variants have been found but the full-length nature of these variants has not been determined. [provided by RefSeq, Dec 2012]
Description: This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis. One version of pediatric acute myeloid leukemia is the result of a reciprocal translocation between chromosomes 11 and X, with the breakpoint associated with the genes encoding the mixed-lineage leukemia and septin 2 proteins. This gene encodes four transcript variants encoding three distinct isoforms. An additional transcript variant has been identified, but its biological validity has not been determined.
Description: This gene is a member of the septin family involved in cytokinesis and cell cycle control. This gene is a candidate for the ovarian tumor suppressor gene. Mutations in this gene cause hereditary neuralgic amyotrophy, also known as neuritis with brachial predilection. A chromosomal translocation involving this gene on chromosome 17 and the MLL gene on chromosome 11 results in acute myelomonocytic leukemia. Multiple alternatively spliced transcript variants encoding different isoforms have been described.
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is highly expressed in brain and heart. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. One of the isoforms (known as ARTS) is distinct; it is localized to the mitochondria, and has a role in apoptosis and cancer.
Description: This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19.
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Description: This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced.
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
