Biovision DL-Serine Standard Curve

DL-seriinianalüüsi komplekt (fluoromeetriline)

KIRJELDUS

Seriin kuulub 20 looduslikult esineva aminohappe hulka, mida kõik organismid kasutavad valkude biosünteesis. Ühe kiraalse keskosa korral võib seriin eksisteerida kahe stereoisomeerina (L-seriin ja D-seriin). Lisaks oma funktsioonile valgu konstrueeriva plokina toimib L-seriin paljude väga oluliste biomolekulide eellasena koos fosfatidüülseriini, sfingomüeliini ning aminohapete glütsiini ja tsüsteiiniga.

DL-Serine Standard Curve
DL-Serine Standard Curve

Mitteproteogeenset isomeeri D-seriini saab imetajate meelest sünteesida ka L-seriinist. Kui enamik D-aminohappeid on imetajatel ainult vihjekogustes, siis D-seriin on oluline neurotransmitter, mis toimib sünaptiliste N-metüül-D-aspartaadi (NMDA) sortide glutamaadiga ühendatud ioonikanalite kaasagonistina. D-seriini metabolismi halvenemine võib kaasa aidata mitmetele neuropsühhiaatrilistele probleemidele koos melanhoolia, skisofreenia, epilepsia ja dementsusega. BioVisioni DL-seriinianalüüsi pakett võimaldab iga L- ja D-seriini kvantifitseerida orgaanilistes vedelikes ja kudedes. Analüüs põhineb L-seriini muundamisel D-seriiniks, mis metaboliseeritakse vaheproduktiks, mis seejärel oksüdeeritakse ja reageerib sondiga, et tekitada püsiv fluorofoor (Ex/Em = 539/587 nm). Proove võib jagada ja analüüsida samaaegselt iga D-seriini ja täieliku DL-seriini kvantifitseerimiseks. Analüüsi lihtsalt ei mõjuta erinevate aminohapete füsioloogilised kontsentratsioonid, see on suure läbilaskevõimega kohandatav ja võib tuvastada alla 1 μM seriini

DL Serine Standard Curve
DL Serine Standard Curve
Kassi # +mõõtmine Olgu 743-100
Mõõtmine 100 testi
Paketi kokkuvõte • Tuvastamismeetod- Fluorestsents (Ex/Em 535/587 nm)
• Tarkvara- Seriini mõõtmine orgaanilistes proovides, mis meenutavad seerumit, plasmat, CSF-i jne.
Avastamise metoodika Fluorestsents (Ex/Em 535/587 nm)
Liigid Reaktsioonivõime Imetaja
Eesmärgid Seriini hindamine erinevates orgaanilistes proovides
Valikud ja eelised • Lihtne, kiire ja mugav test
• Tuvastamispiirang: 1 µM
• Väga spetsiifiline, ei tööta koos erinevate normaalsete ja mittestandardsete aminohapetega
Paketi elemendid • Seriinianalüüsi puhver
• Sondi vastus
• Seriini ratsemaasi ensüümikombinaat
• D- seriini ensüümikombinaat
• Arendaja ensüümikombinaat
• Mustri puhastamise kombineerija
• D-seriin Tavaline
Säilitamise olukorrad -20 ° C
Kohaletoimetamise olukorrad Geelipakett
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